PEPSENS

Biomimetic peptide scaffolds affording tunable biosensor interfaces

 

 

 


Abstract

Biosensors capable of sensing and responding to small molecules have wide-ranging applications, but despite such broad utility, no single strategy for the construction of biosensors has proven sufficiently generalizable to gain widespread use. The project aim is to design novel biomimetic peptide scaffolds generating biosensor interfaces, which can be tuned as common biorecognition interface for different transducers. The modular peptide-based sensors proposed will exploit the conductive and self-assembling properties of short helical peptides along with their ability to easily form amide, thioether and ester bonds in the presence of cross-linking reagents. This approach makes it possible to produce a wide variety of biosensors for different small molecules detection with application in clinical laboratory, environmental and food quality monitoring.


Contract: code PN-III-P4-ID-PCE-2016-0288, no. 20 from 12/07/2017

Acronym: PEPSENS

Contracting authority: UEFISCDI

Duration of the project: 30 months (2017-2019)

Project Director:

Prof. dr. Camelia Bala
University of Bucharest
Department of Analytical Chemistry
Tel./Fax: +4021 410 48 88
Email: camelia.bala@chimie.unibuc.ro

Team members:

Drd. Madalin Danila
ACS dr. Lucian Gabriel Zamfir
ACS dr. Oana Maria Dumitrascu
Lector dr. Mihaela Elena Puiu
Conf. Dr. Lucian Rotariu

Objectives:
-         Developing peptide self-assembled monolayers,
-         Grafting the low molecular weight compounds onto peptide assembled monolayers on gold,
-         Characterization and optimization of biosensors response.

Expected results:

Biosensors for application in clinical laboratory, environmental and food quality monitoring.

Dissemination:

Molecular Recognition: Versatility and Challenges in the Design of Nanobiosensors for Food Security, M. Puiu, C. Bala, in Trends in Food Safety and Protection, Edited by Ravishankar Rai V. and Jamuna A. Bai, Taylor & Francis Ltd., 2017, 51-82

Peptide-based biosensors: From self-assembled interfaces to molecular probes in electrochemical assays, M. Puiu, C. Bala, Bioelectrochemistry, 120, 2018, 66-75.

C. Bala, XXIV International Symposium on Bioelectrochemistry and Bioenergetics, 3-7 July, 2017, Lyon, France (invited lecture)

C. Bala, The 8th International Workshop on Surface Modification for Chemical and Biochemical Sensing Programme, Żelechów, Poland, 3 - 7 November 2017 (Key note)

G. Danila, L. G. Zamfir, M. Puiu, C. Bala, The 8th International Workshop on Surface Modification for Chemical and Biochemical Sensing Programme, Żelechów, Poland, 3 - 7 November 2017 (poster)

 

Last updated at: January 18, 2018.